Development and Universal Application of Artificial Intron Technology for the Generation of Conditional Alleles in Mice
Speakers: Stephane Pelletier, Ph.D., Director of the Indiana University Genome Editing Center
Hosted by: Indiana University Genome Editing Center
Description: Engineering conditional alleles in mice has historically posed a challenge. However, in a recent proof-of-concept study, we made significant progress using a novel technology that employs a short artificial intron to create conditional alleles in mice. This innovative method, based on the Cre-loxP system, involves inserting a short DNA segment containing basic intron elements flanked by loxP sites within an exon. Under normal circumstances (without Cre), this DNA segment functions as an intron and is efficiently removed during splicing, allowing normal gene expression. Conversely, in the presence of Cre, the intronic sequences are specifically excised, leaving a short DNA segment within the exon. Consequently, this altered DNA segment evades recognition by the splicing machinery, resulting in gene inactivation. Our findings expand the potential of this approach for engineering conditional alleles in mice, and ongoing investigations aim to evaluate its universality.
This summit will address environmental triggers related to asthma and housing, the perils of second- and third-hand smoking, and the exploration of myths surrounding exercise and asthma.
Register here.
CADI meets on the second Thursday of odd months.
The Cardiovascular and Diabetes Coalition of Indiana (CADI) is working to help Hoosiers address the burden of cardiovascular diseases, diabetes, and stroke through education, collaboration, awareness, advocacy, and access to care. CADI uses its long-standing history of engaging various partners throughout the state in service of this work. The coalition has successfully been able to develop and launch the Better Together Plan, Indiana’s first statewide strategic plan to address cardiovascular disease, diabetes and stroke together.
To join the coalition, contact Aliya Amin at ajamin@iu.edu.
HHWA meets on the second Tuesday of odd months.
The Hoosier Health and Wellness Alliance (HHWA) seeks to increase positive health outcomes within Indiana by providing the support and effective strategies needed to optimize local impact. Embracing values of collaboration, education and food equity the coalition continues to work on capacity building efforts around the built environment, innovative ways to share the expertise of members, and an increased level of community engagement.
To join the coalition, contact Antonia Sawyer at afsawyer@iu.edu.
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Development and Universal Application of Artificial Intron Technology for the Generation of Conditional Alleles in Mice
Speakers: Stephane Pelletier, Ph.D., Director of the Indiana University Genome Editing Center
Hosted by: Indiana University Genome Editing Center
Description: Engineering conditional alleles in mice has historically posed a challenge. However, in a recent proof-of-concept study, we made significant progress using a novel technology that employs a short artificial intron to create conditional alleles in mice. This innovative method, based on the Cre-loxP system, involves inserting a short DNA segment containing basic intron elements flanked by loxP sites within an exon. Under normal circumstances (without Cre), this DNA segment functions as an intron and is efficiently removed during splicing, allowing normal gene expression. Conversely, in the presence of Cre, the intronic sequences are specifically excised, leaving a short DNA segment within the exon. Consequently, this altered DNA segment evades recognition by the splicing machinery, resulting in gene inactivation. Our findings expand the potential of this approach for engineering conditional alleles in mice, and ongoing investigations aim to evaluate its universality.
This summit will address environmental triggers related to asthma and housing, the perils of second- and third-hand smoking, and the exploration of myths surrounding exercise and asthma.
Register here.
CADI meets on the second Thursday of odd months.
The Cardiovascular and Diabetes Coalition of Indiana (CADI) is working to help Hoosiers address the burden of cardiovascular diseases, diabetes, and stroke through education, collaboration, awareness, advocacy, and access to care. CADI uses its long-standing history of engaging various partners throughout the state in service of this work. The coalition has successfully been able to develop and launch the Better Together Plan, Indiana’s first statewide strategic plan to address cardiovascular disease, diabetes and stroke together.
To join the coalition, contact Aliya Amin at ajamin@iu.edu.
HHWA meets on the second Tuesday of odd months.
The Hoosier Health and Wellness Alliance (HHWA) seeks to increase positive health outcomes within Indiana by providing the support and effective strategies needed to optimize local impact. Embracing values of collaboration, education and food equity the coalition continues to work on capacity building efforts around the built environment, innovative ways to share the expertise of members, and an increased level of community engagement.
To join the coalition, contact Antonia Sawyer at afsawyer@iu.edu.