Macromolecular Crystallography Facility Integrated with the Crystallization Automation Facility (MCF/CAF)

/Macromolecular Crystallography Facility Integrated with the Crystallization Automation Facility (MCF/CAF)/

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Macromolecular Crystallography Facility Integrated with the Crystallization Automation Facility (MCF/CAF)

Affiliation(s):  Indiana University Bloomington

Executive Director:   Charles E. Dann, Ph.D.

Website:  http://www.indiana.edu/~iumcf/

Description:
The Macromolecular Crystallography Facility at Indiana University, Bloomington welcomes Researchers interested in Structural Biology. Over the past several years there has been a rapid increase on our campus of research into biological structures, spanning from DNA (RNA) protein complexes, viral particles/capids, and protein macromolecules. With this ever growing interest, the MCF provides a home source for X-Ray diffraction data collection from macromolecular crystals and computational support for structure determination and refinement. The Facility houses a Rigaku RUH-3R X-Ray generator with two RaxisIV++ image plate detectors. In addition, we are a member institution in the Molecular Biology Consortium, which allows for easy, remote synchrotron access on beamline 4.2.2 at the Advanced Light Source (ALS), a part of Lawrence Berkeley National Labs in Berkeley, California. This facility was founded to facilitate and encourage research endeavors in structural biology and to provide a centralized resource for training and education in latest crystallographic and structural biology techniques. Furthermore, the facility as a whole proudly introduces the Crystallization Automation Facility (CAF) comprised of three new instruments that provide high throughput robotic automated crystallization. Given the capacity of this syetm, we envision that other research organizations in the region will also benefit from use of the CAF.

Policies: Services Offered:
  • The Alchemist is designed to generate customized crystallization screens based on initial crystallization “hits” to yield diffraction quality crystals.
  • Phoenix HT / RE, a drop setting robot that distributes user samples and potential crystallants in 96-3 format plates (up to 288 trials per plate).
  • The Minstrel HT/UV coupled with two Gallery 700 incubators allows for user-free storage and imaging of all crystallization trails. Image data are stored on a server that can be accessed remotely via the CrystalTrak Web software.
Keywords for this Core:
x-ray crystallography, crystallography, macromolecular structure, crystal structure, crystallization, imaging, structural biology, protein complexes, viral particles / capids, protein macromolecules, structure determination and refinement

Contact Information:
Giovanni Gonzalez-Gutierrez
Email This Core
812-856-7505

Order Form:


By |2018-10-31T10:12:00-04:00October 31st, 2018|Comments Off on Macromolecular Crystallography Facility Integrated with the Crystallization Automation Facility (MCF/CAF)

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