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Proteomics Facility
Proteomics Facility
Affiliation:
Purdue University
Director:
Dr. Uma K Aryal
Manager:
Dr. Jackeline Franco
Website:
Description:
The Purdue Proteomics Facility (PPF) provides innovative state-of-the-art LCMS/MS analysis of proteins in clinical, environmental and other biological samples. As a shared facility of the Bindley Bioscience Center (BBC) in Discovery Park of Purdue University, the facility enables both targeted and global analysis of proteins, their post-translational modifications and analysis of protein complexes and protein-protein interactions. Coupled with different chromatographic separation techniques, modern mass spectrometric instrumentation, and advanced scientific and bioinformatics expertise, the facility provides unique opportunities for researchers at Purdue and beyond to perform quantitative analysis of proteins in complex biological samples using both global, as well as, targeted proteomic approaches.
Policies:
Services:
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Sample preparation including cell lysis, protein extraction, in-solution and in-gel protein digestion, peptide clean-up
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LC-MS/MS analysis for protein identification, label and label free quantitation, global and targeted proteomics
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Analysis of post-translationally modified proteins and peptides
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MALDI TOF MS analysis of intact protein and peptides
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Gel filtration (SEC) and ion exchange chromatography (IEX) coupled with quantitative MS profiling for the analysis of protein complexes
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Proteomics and phosphoproteomics method development and consultation
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Bioinformatic consultation and support for database searches for protein identification, label and label free quantitation, and data interpretation
Contact:
- Uma Aryal : uaryal@purdue.edu